Qubit® dsDNA BR Assay Kit. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. PicoGreen dsDNA quantitation reagents are. • Specific for dsDNA in the presence of RNA. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Rapidly detect as little as 0. Quant-iT RNA BR (Broad Range) Assay Kit. 2–100 ng, the fluorescence signal is linear with DNA. Cloning, expression and proteins purification. Rapidly detect as little as 0. This kit contains. Specifications Documents Scientific Resources Application Notes Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Materials & Equipment Required But Not Provided • Pipettors - 5 L - 1200 L. Catalog Number. Quantitation using Quant-iT Assay Kits. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. Picogreen assays are compatible with most microplate readers and fluorometers. This protocol is a simplified and condensed version of. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. assay. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen) May 22, 2007. 5. 0 ml 200x in DMSO b. Price from $9. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. Figure 2. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. Rapidly detect as little as 0. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. High tolerance for contaminants. Sequencing libraries were built using Illumina's TruSeq Nano DNA LT Library Prep Kit, and the NovaSeq 6000 SP Reagent Kit (500 cycles) was used. For running these libraries on the MiSeq and HiSeq, please make sure you read the supplementary methods of Caporaso et al. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. For duplicate-well droplet. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. (The same tips can be used. 0 mL to 200 uL to fit a 96-well microplate format. ITSkabir). The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. 适用于（设备）. PicoGreen dsDNA quantitation reagents are. The Qubit 1X dsDNA BR Standards includes standards at two concentrations, 0 and 100 ng/µL. Using this data as a starting point, the optimal conditions (e. Quantitation Range. Picogreen assays are compatible with most microplate readers and fluorometers. Begin typing to search, use arrow keys to navigate, Enter to select. Catalog Number. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. Catalog Number. 0 fluorometer—provides state-of-the-art reagents for sensitive and selective quantitation of DNA, RNA or protein samples using a standard fluorescence microplate reader or the Qubit 2. The PicoGreen is sticking to the sides of the cuvette or assay plate. PicoGreen dsDNA quantitation reagents are. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. Therefore, fluorescence spectroscopy using PicoGreen is more useful than UV spectroscopy for evaluating the template activity of DNA for PCR. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. In addition, the concentration of DNA obtained from the 1860 tomato samples was measured fluorometrically using Quant-iT™ PicoGreen™ dsDNA Assay Kit (hereafter PicoGreen, Thermo Scientific, Waltham, MA, USA) and a 96-wells plate reader VICTOR3 1420 (PerkinElmer, Waltham, MA, USA) equipped with an excitation filter F485 and. • Suitable for use in 96- and 384-well plate formats. Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. No. 2 – 100 ng. 995 mL TE for the . Because the PicoGreen dye is an extremely sensitive detection reagent for dsDNA, it is imperative that the TE buffer is free of contaminating nucleic acids. 2023-11. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. The PicoGreen reagent was freshly prepared by diluting 15 μL reagent with 2 mL of TE buffer (~ 133X) and stored in the dark for no longer than 2 hours. Bioz Stars score: 86/100, based on 1 PubMed citations. Prepare the assay. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. Picogreen assays are compatible with most microplate readers and fluorometers. 5 mm in the Eppendorf BioSpectrometer fluorescence, PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. Facebook. 99. Rapidly detect as little as 0. Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. The assay is a homogeneous, mix-and-read format where the fluorescence is proportional to analyte present. Figure 2. An aliquot (1. • Suitable for use in 96- and 384-well plate formats. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. The assay is performed at room temperature, and the signal is stable for 3 hours. PCR products were quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Microplate reader (Bio-Tek, FLx800), and then mixed according to the amount of data required for each sample. Table 2 summarizes results of the. Table 2 summarizes results of the. 99. 2022. This protocol is a simplified and condensed version of. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 25 pg/uL 的 dsDNA。 该定量试剂盒在三个数量级内呈线性，几乎无序列依赖性，可让您准确测定多种来源的 DNA，包括基因组 DNA、病毒 DNA、小提 DNA 或 PCR 扩. Picogreen assay kit (Invitrogen, P7589) a. Unit Size. Picogreen assays are compatible with most microplate readers and fluorometers. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. P7589. 326,933 327K. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Rapidly detect as little as 0. Rapidly detect as little as 0. 99. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Quant-it Picogreen® dsDNA assay kit (ref P11496) was obtained from Thermofisher scientific. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Signal measurements of all samples were performed using the EnVision 2105 Multimode Plate Reader (PerkinElmer, Waltham, MA,. Briefly, 2 µL of sample was diluted in 498 µL TE buffer. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Rapidly detect as little as 0. Technical note | Quant-iT and Qubit 1X dsDNA assaysthe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Briefly, 2 µL of sample was diluted in 498 µL TE. 8 Measurement of growth factor content in. Q33130). The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Significantly increased sensitivity over absorbance at 260nm (NanoDrop® spectrophotometer) for those samples that are low in concentration. 激发/发射. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. Picogreen assays are compatible with most microplate readers and fluorometers. The reagent must be completely thawed before using it by bringing it to room temperature. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. This protocol is a simplified and condensed. • Suitable for use in 96- and 384-well plate formats. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). 99 to $1999. Figure 8. The Quant-iT™ dsDNA BR assay can be adapted for use in cuvettes or 384-well microplates. Quant-iT PicoGreen dsDNA Assay Kits and Reagents are designed to selectively detect dsDNA from a wide variety of sources, even in presence of ssDNA, RNA, and free. 3 Q33120 39. . The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. Rapidly detect as little as 0. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. Quant It Picogreen Dsdna Assay Kit Reagents, supplied by Thermo Fisher, used in various techniques. Catalog Number. Mit dem PicoGreen dsDNA. The ITS protocol detailed here is designed to amplify fungal microbial eukaryotic lineages using paired-end community sequencing on the Illumina platform with primers ITS1f-ITS2 (EMP. The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. Picogreen assays are compatible with most microplate readers and fluorometers. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. 1. The Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). P7589. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. dsDNA Assay Kit: Invitrogen Quant-iT PicoGreen dsDNA Assay Kits and dsDNA Reagents, spectrophotometer, qPCR Quantification Kit: Roche KAPA Library Quantification Kits — Complete Kit (Universal), and qPCR Plate Reader: LightCycler 480 Instrument II. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific,. Alternatively, purified RCA products can be quantitated by measuring the absorbance at 260 nm or by NanoDrop®. . The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. dsDNA assays. Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA. Bioz Stars score: 86/100, based on 1 PubMed citations. Barcoded amplicons were pooled in. Product Type. Rapidly detect as little as 0. It is specific for dsDNA in the presence of equimolar amounts of RNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. PicoGreen dsDNA quantitation reagents are. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. Genomic DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit a. Quant-iT PicoGreen dsDNA Reagent. Picogreen assays are compatible with most microplate readers and fluorometers. Pico Green dsDNA Assay 2 03/04/2019The assay was performed according to the initial MPO-DNA complex ELISA protocol, but blocking buffer, sample diluent and detection antibody diluent were replaced by an in-house blocking buffer. Rapidly detect as little as 0. DNA standard curve. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. The fluorescence spectra of dsDNA stained with PicoGreen when in solution equimolar concentration of RNA was also present ([DNA]/[RNA] = 1, red lines in figure 4) did notObtenga mediciones precisas de la concentración de ADNbc en un amplio rango dinámico con los kits de ensayo de ADNbc y reactivos de ADNbc Quant-iT Picogreen. Free dye. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. Using this data as a starting point, the optimal conditions (e. The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA, with a linear range from ~1 – 3,000 pg (depending on instrument capability). Dilute from 20xTE stock (supplied in the PicoGreen kit). • Quant-iT dsDNA Assay Kit, high sensitivity (Cat. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. i) 110 sample x 150uL =16. High linearity and sensitivity over a broad DNA range. 2-200 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iTTM DNA Assay Kit, Broad Range 2-1000 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iT™ DNA Assay Kit, High Sensitivity 0. 3ng/mL，DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). • Specific for dsDNA in the presence of RNA. 2 to 100 ng) Assay Kit were used with a Qubit 3. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). Module in combination with PicoGreen® dye allows the direct quantitation of dsDNA in as little as 100 µL total volume. Figure 1. Reagent Search & Data Services Menu Reagent Search. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Quant-iT PicoGreen dsDNA Reagent. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. According to the obtained results, the assay presents a linear correlation in a wide range of 0. Below are some easy steps to perform quantification in plates with Pico488 quantification kit by Lumiprobe (analog of Quant-iT® PicoGreen® dsDNA Assay Kit). Description. The PicoGreen dsDNA quantitation assay can be used when selectively detecting as little as 25 pg/ml of dsDNA with free nucleotides, RNA and ssDNA present, having minimal impact on the intensity of. Alternatively, purified RCA products can be quantitated by measuring the absorbance at 260 nm or by NanoDrop®. Quant-iT™ PicoGreen ® dsDNA Assay Kit. dsDNA and PicoGreen analyzed using the Modulus and the Blue Fluorescence Optical Kit. The assay is highly selective for double- stranded DNA over RNA, and in the. No. 0. Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). Bioz Stars score: 86/100, based on 1 PubMed citations. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. 04 corresponds to 2 μ g/mL dsDNA solution. 2. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Picogreen kinetic assay was based on polymerase activity of SARS nsp12 in complex with nsp7L8, which. quant it picogreen dsdna kit - by Bioz Stars , 2023-09. Further to the Quant-iT dsAssay kit (PicoGreen®), the Qubit dsDNA HS Assay kit offers an additional option for quantify-ing dsDNA samples of low concentration on the BioSpec-trometer fluorescence with high specificity and sensitivity. 5 ng/ml ~ 1,000 ng/ml 까지 정비례. Optimized for fluorescence microplate readers. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. DNA samples with a concentration determined by 260 nm absorbance higher than 100 ng/mL were diluted. Materials: 1. Prepare the rest of the standards , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. Unit Size. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. The Quant-iTTM PicoGreen® dsDNA Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures. Rapidly detect as little as 0. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. The Qubit dsDNA BR Assay kit contains a highly selective enhancing reagent specific for quantifying dsDNA using volumes as low as 1 µL in a standard 96-well microplate format. Optimized for fluorescence microplate readers. 製品番号（カタログ番号）： P7581. Quant-iT PicoGreen dsDNA Assay Kit a. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). Calibrator MPO concentration was quantified with a commercially available assay (Human Myeloperoxidase Quantikine ELISA Kit, Bio-Techne, Minneapolis, MN) according to the manufacturer’s recommendations. P7589) was used to quantify DNA by fluorescence. It requires the most user preparation and calculation, but can be adapted for cuvettes or plates. This protocol is a simplified and c. Quantitation Range. Los ensayos Picogreen son compatibles con la mayoría de los lectores de microplacas y fluorómetros. 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. Product Type. Bioz Stars score: 86/100, based on 1 PubMed citations. Rapidly detect as little as 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Catalog number: P7581. Common contaminants such as salts, free nucleotides, solvents,Product Description. Working PicoGreen reagent was prepared by diluting the concentrated DMSO-PicoGreen stock solution, provided in the PicoGreen kit, 1:200 with TE according to the kit instructions. The assay is highly selective for double-stranded DNA (dsDNA) over RNA (Figure 1, page 7) and is accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. 0 ml 200x in DMSO b. Qubit™ dsDNA Quantification Assay Kits. • Suitable for use in 96- and 384-well plate formats. • Specific for dsDNA in the presence of RNA. 3,201 3. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 8. The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. coli rRNA (Δ), or a 1:1 mixture of DNA and RNA (∎) were assayed in the Quant-iTTM dsDNA HS assay. Sample fluorescence was. to each well. PicoGreen dsDNA quantitation reagents are. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. Picogreen assays are compatible with most microplate readers and fluorometers. Q33120) • Quant-iT dsDNA Assay Kit, broad range (Cat. is 1 pg/μL – 5 ng/μL so the sensitivity of this kit can be rated as Ultra High (UHS). 产品概述. AccuBlue® RNA Quantitation Kit Details. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM 製品番号（カタログ番号）： P11495. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). 3. 製品概要. Rapidly detect as little as 0. Picogreen assays are compatible with most microplate readers and fluorometers. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). The assays are many times more sensitive than conventional UV absorbance and can be optimized for different concentration ranges. 21 DiB) [22]. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. PicoGreen dsDNA quantitation reagents are. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Suitable for use in 96- and 384-well plate formats. PicoGreen dsDNA quantitation reagents are. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The range of linearity for DNA concentration measurements. DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). The assay is highly selective for RNA over double-stranded DNA, and provides a dynamic range of 5-100 ng of RNA. PicoGreen®試薬アッセイ. P11496). (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. For Research Use Only. Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. Picogreen assays are compatible with most microplate readers and fluorometers. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. 9. quant it picogreen dsdna assay kit , 2023-10. Unit Size. This sensitivity exceeds that achieved with the Hoechst 33258. You will need 100 ul diluted PicoGreen per well containing 100 ul sample. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. 7 Q33231 6 1,000 41. A12216) Metabolic (glucose) • Amplex Red Glucose/Glucose Oxidase Assay Kit (Cat. using a PicoGreen assay by the company life technologies (Quant-iT™ PicoGreen dsDNA Reagent and Kits). Table 2 summarizes results of the. Automation of the Quant-iT™ PicoGreen® dsDNA Assay Kit on the Fluent. 文件. 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Equal amounts (100 µL) of working PicoGreen quantitation reagent were mixed and incubated for 10 minutes at room temperature, protected from light. 2 - 100 ng/ml 480/520 NaCl, MgCl 2Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9. 95 mL TE for the . DNA. (D–G) TLR stimulation was measured using a HEK293-TLR reporter. The original sequencing data. • Suitable for use in 96- and 384-well plate formats. quant it picogreen dsdna quantitation assay kit - by Bioz Stars , 2023-09. Rapidly detect as little as 0. 2. Skip to main list 美谷分子仪器（上海）有限公司. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT PicoGreen dsDNA Assay Kit. 86100 stars. For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2. Quantitation Range. ZERO BIAS - scores, article reviews, protocol conditions and moreSelectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. When 5–500 pg salmon dsDNA or 3–1000 pg control dsDNA (calf thymus dsDNA standard) is added to AccuClear a linear response is observed (Fig. 8 List of Tables Molecular Probes amine-reactive dyes—Table 1. methods for the quantitation of DNA: the Invitrogen™ Quant-iT™ PicoGreen™ dsDNA assay and the Invitrogen™ Qubit™ dsDNA assays. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Related applications: Nucleic Acid Quantitation. Q33130) Metabolic (cholesterol) • Amplex Red Cholesterol Assay Kit (Cat. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Picogreen assays are compatible with most microplate readers and fluorometers. Lambda DNA was used to generate the. The differences in mechanical testing, cell proliferation in PrestoBlue™ and Quant-iT™ Picogreen™ dsDNA Assay were evaluated using t-test and one-way ANOVA, followed by Tukey’s post-test with α = 0. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%).